Coding

Part:BBa_K2923012:Design

Designed by: Kateryna LEN   Group: iGEM19_Strasbourg   (2019-10-13)


PP7 coat protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 351
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PP7cp was amplified with overlapping sequences for Gibson assembly from BH3 plasmids


Source

Pseudomonas phage PP7

References

Berry, K.E., and Hochschild, A. (2018). A bacterial three-hybrid assay detects Escherichia coli Hfq-sRNA interactions in vivo. Nucleic Acids Research 46